English Intern
Institut für Virologie und Immunbiologie

Imaging

Core Unit for Confocal Microscopy

Contact: 

Dr. Elita Avota

Tel. +49 931 31 81521

Email

or

Dr. Bhupesh Prusty

Tel. +49 931 31 88067

Email

 

External users please contact via email. 

Facility fees per started hour:

1st hour: 25,00 €
2nd hour: 20,00 €
3rd hour: 15,00 €
4th hour plus: 10,00 €/hour

Billing will be on a quarter-yearly basis. Prices are in accordance with DFG guidelines and have been approved by the boards of the core facility and the IZKF.

Confocal Laser Scanning Microscope: Zeiss LSM 780

Inverted Microscope (Cell Observer Z.1)

XL incubator for live cell imaging (S2 level)

Laser lines: 405, 458/488/514, 561, 594 and 633 nm
Detectors: high sensitive GaAsP Array detector and 2 PMTs, 1 T-PMT (DIC)
Objectives: 10x, 20x, 40x Oil (NA 1.4), 63x Oil (NA 1.4)

Software Options: spectral imaging and linear unmixing, colocalisation, 3D reconstruction, mosaic and stitching, Time lapse, FRET
Additional: Huygens deconvolution software

Exemplified Applications

- Multi-color analysis

- Immunofluorescence analysis of fixed cells and tissue

- Live cell imaging: labeling of cellular compartments and structures, cell/cell interactions, fluorescence-based activation assays, chemotaxis

Service

- Introduction and training on the microscopic system
- General advice on diverse fluorescence techniques, cell biological assays, live cell microscopy and image analysis