Deutsch Intern
    Institute for Virology and Immunobiology


    Core Unit for Confocal Microscopy


    Dr. Christine Krempl



    0049-(0)931-31-81 549 (office)

    External users please contact via email. 

    Facility fees per started hour:

    1st hour: 25,00 €
    2nd hour: 20,00 €
    3rd hour: 15,00 €
    4th hour plus: 10,00 €/hour

    Billing will be on a quarter-yearly basis. Prices are in accordance with DFG guidelines and have been approved by the boards of the core facility and the IZKF.

    Inverted Confocal Laser Scanning Microscope: Zeiss LSM 510 Meta
    laser lines: 458/488/514 nm, 543 nm, 633 nm
    2 PMTs, 1 32-channel Meta detector, 1 Transmission-PMT
    live cell imaging: heated incubation chamber

    Sample Applications
    -microscopic analysis of fixed and live cell cultrues, tissue
    -z-stacking and 3D reconstruction (e.g. visualisation of the immunological synapse)
    -live cell imaging: chemotaxis, cell migration, cell interactions, flourescence-based
     activation assays, labeling of cells and cellular compartments

    -provide training on the instrument
    -general advice on image analysis, live cell microscopy, flourescence techniques and measurements
    -assistance on data analysis
    -colocalisation, image restoration (Huygens deconvolution) 3D reconstruction,
     cell tracking


    Research interests
    Modulation of T lymphocyte polarity and cytoskeleton architecture during immunosuppressive processes and analysis of associated signaling mechanisms