Imaging
Core Unit for Confocal Microscopy
Contact:
Dr. Christine Krempl
Tel.:
0049-(0)931-31-81 549 (office)
External users please contact via email.
Facility fees per started hour:
1st hour: 25,00 €
2nd hour: 20,00 €
3rd hour: 15,00 €
4th hour plus: 10,00 €/hour
Billing will be on a quarter-yearly basis. Prices are in accordance with DFG guidelines and have been approved by the boards of the core facility and the IZKF.
Instrument
Inverted Confocal Laser Scanning Microscope: Zeiss LSM 510 Meta
laser lines: 458/488/514 nm, 543 nm, 633 nm
2 PMTs, 1 32-channel Meta detector, 1 Transmission-PMT
live cell imaging: heated incubation chamber
Sample Applications
-microscopic analysis of fixed and live cell cultrues, tissue
-z-stacking and 3D reconstruction (e.g. visualisation of the immunological synapse)
-live cell imaging: chemotaxis, cell migration, cell interactions, flourescence-based
activation assays, labeling of cells and cellular compartments
Service
-provide training on the instrument
-general advice on image analysis, live cell microscopy, flourescence techniques and measurements
-assistance on data analysis
-colocalisation, image restoration (Huygens deconvolution) 3D reconstruction,
cell tracking
Research interests
Modulation of T lymphocyte polarity and cytoskeleton architecture during immunosuppressive processes and analysis of associated signaling mechanisms
