piwik-script

English Intern
    Institut für Virologie und Immunbiologie

    Core Unit for Confocal Microscopy

    Contact: 

    Dr. Christine Krempl

    CKrempl@vim.uni-wuerzburg.de

    Tel.: 

    0049-(0)931-31-81 549 (office)

    External users please contact via email. 

    Facility fees per started hour:

    1st hour: 25,00 €
    2nd hour: 20,00 €
    3rd hour: 15,00 €
    4th hour plus: 10,00 €/hour

    Billing will be on a quarter-yearly basis. Prices are in accordance with DFG guidelines and have been approved by the boards of the core facility and the IZKF.

    Confocal Laser Scanning Microscope: Zeiss LSM 780

    Inverted Microscope (Cell Observer Z.1)

    XL incubator for live cell imaging (S2 level)

    Laser lines: 405, 458/488/514, 561, 594 and 633 nm
    Detectors: high sensitive GaAsP Array detector and 2 PMTs, 1 T-PMT (DIC)
    Objectives: 10x, 20x, 40x Oil (NA 1.4), 63x Oil (NA 1.4)

    Software Options: spectral imaging and linear unmixing, colocalisation, 3D reconstruction, mosaic and stitching, Time lapse, FRET
    Additional: Huygens deconvolution software

    Exemplified Applications

    - Multi-color analysis

    - Immunofluorescence analysis of fixed cells and tissue

    - Live cell imaging: labeling of cellular compartments and structures, cell/cell interactions, fluorescence-based activation assays, chemotaxis

    Service

    - Introduction and training on the microscopic system
    - General advice on diverse fluorescence techniques, cell biological assays, live cell microscopy and image analysis