Methodik

Techniques

 

Tissue culture mainly involving primary T cells and dendritic cells generated from bone marrow or human monocytes

Establishment of 3D cultures (tracheal model)

Generation and amplification of recombinant measles viruses (virus bindung, uptake and trafficking studies)

Cloning of expression vectors and mutagenesis

Genetic manipulation of cell lines and primary cells (DNA and siRNA transfection, lentiviral transduction for transgene expression or gene silencing)

RT-PCR

Western-Blots

Sphingomyelinase activity assays (and other enzymatic assays)

FACScanning and FACSsorting

Functional assays:
Cytokine measurements (ELISA, FACS)

Endocytosis assays

Adhesion and Migration assays

Proliferation assays (3H incorporation, CFSE labeling)

Viability assays

Confocal microcopy

Live cell imaging