Methodik
Techniques
Tissue culture mainly involving primary T cells and dendritic cells generated from bone marrow or human monocytes
Establishment of 3D cultures (tracheal model)
Generation and amplification of recombinant measles viruses (virus bindung, uptake and trafficking studies)
Cloning of expression vectors and mutagenesis
Genetic manipulation of cell lines and primary cells (DNA and siRNA transfection, lentiviral transduction for transgene expression or gene silencing)
RT-PCR
Western-Blots
Sphingomyelinase activity assays (and other enzymatic assays)
FACScanning and FACSsorting
Functional assays:
Cytokine measurements (ELISA, FACS)
Endocytosis assays
Adhesion and Migration assays
Proliferation assays (3H incorporation, CFSE labeling)
Viability assays
Confocal microcopy
Live cell imaging