Core Unit For Confocal Microscopy

Contact: 

Dr. Nora Müller

 

Opens window for sending emailNora.Mueller@uni-wuerzburg.de

 

Tel.: 

0049-(0)931-31-81 475 (office)

0049-(0)931-31-80 662 (lab)

 

 

Confocal Laser Scanning Microscope: Zeiss LSM 780

Inverted Microscope (Cell Observer Z.1)

XL incubator for live cell imaging (S2 level)

Laser lines: 405, 458/488/514, 561, 594 and 633 nm
Detectors: high sensitive GaAsP Array detector and 2 PMTs, 1 T-PMT (DIC)
Objectives: 10x, 20x, 40x Oil (NA 1.4), 63x Oil (NA 1.4)

Software Options: spectral imaging and linear unmixing, colocalisation, 3D reconstruction, mosaic and stitching, Time lapse, FRET
Additional: Huygens deconvolution software

 

Exemplified Applications

- Multi-color analysis

- Immunofluorescence analysis of fixed cells and tissue

- Live cell imaging: labeling of cellular compartments and structures, cell/cell interactions, fluorescence-based activation assays, chemotaxis

 

Service

- Introduction and training on the microscopic system
- General advice on diverse fluorescence techniques, cell biological assays, live cell microscopy and image analysis