Dr. Nora Müller

Tel.  0049-(0)931-201-49973 (office)
Fax. 0049-(0)931-201-49553

Nora.Mueller@uni-wuerzburg.de

Instrument
Inverted Confocal Laser Scanning Microscope: Zeiss LSM 510 Meta
laser lines: 458/488/514 nm, 543 nm, 633 nm
2 PMTs, 1 32-channel Meta detector, 1 Transmission-PMT
live cell imaging: heated incubation chamber

Sample Applications
-microscopic analysis of fixed and live cell cultrues, tissue
-z-stacking and 3D reconstruction (e.g. visualisation of the immunological synapse)
-live cell imaging: chemotaxis, cell migration, cell interactions, flourescence-based
 activation assays, labeling of cells and cellular compartments

Service
-provide training on the instrument
-general advice on image analysis, live cell microscopy, flourescence techniques and measurements
-assistance on data analysis
-colocalisation, image restoration (Huygens deconvolution) 3D reconstruction,
 cell tracking

Research interests
Modulation of T lymphocyte polarity and cytoskeleton architecture during immunosuppressive processes and analysis of associated signaling mechanisms